ACOX2 Antibody

Novus Biologicals | Catalog # NB100-57853

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rabbit (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ACOX2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QWAQKSPTNTQENP corresponding to internal region (near C-Terminus) according to NP_003491.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACOX2 Antibody

Western Blot: ACOX2 Antibody [NB100-57853]

Western Blot: ACOX2 Antibody [NB100-57853]

Western Blot: ACOX2 Antibody [NB100-57853] - (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ACOX2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in human liver lysates (calculated MW of 76.8 kDa band according to NP_003491.1). An additional band of 37 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACOX2

The product of this gene belongs to the acyl-CoA oxidases family. It encodes the branched-chain acyl-CoA oxidase which is involved in the degradation of long branched fatty acids and bile acid intermediates in peroxisomes. Deficiency of this enzyme results in the accumulation of branched fatty acids and bile acid intermediates, and may lead to Zellweger syndrome, severe mental retardation and death in children.

Alternate Names

12-alpha-trihydroxy-5-beta-cholestanoyl-CoA 24-hydroxylase, 12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase, 3-alpha, 7-alpha, acyl-CoA oxidase 2, branched chain, acyl-Coenzyme A oxidase 2, branched chain, BCOX, BRCACOXTHCA-CoA oxidase, BRCOX, EC 1.17.99.3, peroxisomal acyl-coenzyme A oxidase 2,3-alpha, peroxisomal branched chain acyl-CoA oxidase, THCCox, Trihydroxycoprostanoyl-CoA oxidase

Entrez Gene IDs

8309 (Human)

Gene Symbol

ACOX2

UniProt

NP_003491.1 (Human)

Additional ACOX2 Products

Product Documents for ACOX2 Antibody

Certificate of Analysis

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Product Specific Notices for ACOX2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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