Actin (Muscle Specific) Antibody (MSA/953)

Novus Biologicals | Catalog # NBP2-45274

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MSA/953
View all Actin (Muscle Specific) Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide common among human muscle actins

Localization

Cytoplasmic

Marker

Muscle Cell Marker

Specificity

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47662)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Actin (Muscle Specific) Antibody (MSA/953)

Western Blot: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Western Blot: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Western Blot: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - hSKM cell lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Formalin-fixed paraffin-embedded rat stomach stained with muscle specific actin monoclonal antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Skeletal Muscle stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Actin (Muscle Specific) Antibody (MSA/953)

Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] -

Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - An intracellular stain was performed on HepG2 cells with pan Actin [MSA/953] Antibody NBP2-47662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin format of this antibody.

Applications for Actin (Muscle Specific) Antibody (MSA/953)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5-2 ug/ml

Western Blot

0.5-1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.5-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Actin (Muscle Specific)

Alternate Names

ACTA, ACTC, actin, alpha 2, smooth muscle, aorta, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, aortic smooth muscle, ACTSA, alpha-cardiac actin, ASD5, ASMA, Cell growth-inhibiting gene 46 protein, CFTD, CFTD1, CFTDM, CMD1R, CMH11, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3

Gene Symbol

ACTA1

UniProt

P68032, P68133 (Human), P62736 (Human)

Additional Actin (Muscle Specific) Products

Product Documents for Actin (Muscle Specific) Antibody (MSA/953)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Actin (Muscle Specific) Antibody (MSA/953)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Actin (Muscle Specific) Antibody (MSA/953)

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Protocols

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