actin-related protein 2/3 complex subunit 1B Antibody
Novus Biologicals | Catalog # NBP3-23370
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Chicken
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
actin-related protein 2/3 complex subunit 1B synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human actin-related protein 2/3 complex subunit 1B. This peptide sequence is highly conserved in rat, mouse, and chicken Arpc1b, and has some homology to the conserved region in isoform Arpc1a. [UniProt# O15143]
Specificity
The antibody detects a 41 kDa protein corresponding to the molecular mass of actin-related protein 2/3 complex subunit 1B on SDS-PAGE immunoblots of human A431 and Jurkat cells, rat A7r5 and PC12 cells, and mouse C2C12 cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for actin-related protein 2/3 complex subunit 1B Antibody
Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP3-23370] -
Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP3-23370] - Western blot of rat A7r5 (lane 1), human Jurkat (lane 2), rat PC12 (lane 3), human PC3 (lane 4), mouse C2C12 (lane 5), and human A431 cells (lane 6). The blot was probed with rabbit polyclonal anti-Arpc1b (C-terminal region).Applications for actin-related protein 2/3 complex subunit 1B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Immunoprecipitation
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: actin-related protein 2/3 complex subunit 1B
Alternate Names
actin related protein 2/3 complex, subunit 1B (41 kD), actin related protein 2/3 complex, subunit 1B, 41kDa, actin-related protein 2/3 complex subunit 1B, ARC41p41-ARCp40-ARC, Arp2/3 complex 41 kDa subunit, ARP2/3 protein complex subunit p41
Gene Symbol
ARPC1B
Additional actin-related protein 2/3 complex subunit 1B Products
Product Documents for actin-related protein 2/3 complex subunit 1B Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for actin-related protein 2/3 complex subunit 1B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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