ADAMTS5 Antibody - BSA Free

ADAMTS5 Antibody [NBP2-15286] -

Untreated (–) and treated (+) RMS 13 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: ADAMTS5 Antibody - BSA Free [NBP2-15286] -

MiR-23b-3p was the key mediator of osteocyte-derived extracellular vesicles in regulating cartilage metabolism.A A schematic diagram illustrating the separation of extracellular vesicles (EVs) and their enrichment from human tissues and mouse plasma. Heatmaps of relative miRNA differences (B) and enrichment analysis (C). D The miR-23b-3p levels in LS-EVs and HS-EVs (n = 5). E The miR-23b-3p levels in subchondral bone (SCB) osteocytes from WT and Dicer-cKO mice with destabilization of the medial meniscus (DMM) surgery (n = 5). F The miR-23b-3p levels in cartilage and SCB osteocytes from human LS/HS tissues (n = 20). G The miR-23b-3p levels in cartilage and SCB osteocytes from mice with DMM surgery (n = 6). H The pre-miR-23b-3p levels in cartilage and SCB osteocytes from human LS/HS tissues (n = 20). I The pre-miR-23b-3p levels in cartilage and SCB osteocytes from mice with DMM surgery (n = 6). J Alcian blue staining. Scale bar in upper panel, 1 mm. Scale bar in lower panel, 2 mm. K Western blot analysis of COLII, ACAN, SOX9, MMP13 and ADAMTS5 protein expression in primary human and mouse chondrocytes transfected with nc or mimic (n = 3). L The schematic depicted the overexpression of miR-23b-3p in cartilage through intraarticular injection of rAAV5-miR-23b-3p-mcherry (rAAV5-miR-23b-3p) or negative control (rAAV5-nc) in 8-week-old male mice following DMM surgery (n = 8). M The Safranin O images of cartilage. Scale bar in upper panel, 200 μm. Scale bar in lower panel, 100 μm. Data are presented as the mean +/- SD. Data was analyzed by unpaired two-tailed Student’s t-test (D, E, G, I and K) and paired two-tailed Student’s t-test (F and H). Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40399261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ADAMTS5 Antibody - BSA Free [NBP2-15286] -

Regulatory effects of Ept on catabolic and anabolic dynamics in hOACs. (A) Immunofluorescent staining against Col II and aggrecan (ACAN) in Ept or vehicle treated hOACs. (B) PCR results of Col II, ACAN, MMP-13, and ADAMTS5 (n = 3). (C) Representative western blot detection of ADAMTS5, ACAN, MMP-13, Col II, and beta -actin (n = 3). (D) Normalized quantitative data from western blot assay in Ept or vehicle treated hOACs. (E and F) Elisa detection of ADAMTS5 and MMP-13 levels in culture medium (n = 3). Mean +/- SD, p* < 0.05, p** < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36568290), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ADAMTS5 Antibody - BSA Free [NBP2-15286] -

MiR-23b-3p was the key mediator of osteocyte-derived extracellular vesicles in regulating cartilage metabolism.A A schematic diagram illustrating the separation of extracellular vesicles (EVs) and their enrichment from human tissues and mouse plasma. Heatmaps of relative miRNA differences (B) and enrichment analysis (C). D The miR-23b-3p levels in LS-EVs and HS-EVs (n = 5). E The miR-23b-3p levels in subchondral bone (SCB) osteocytes from WT and Dicer-cKO mice with destabilization of the medial meniscus (DMM) surgery (n = 5). F The miR-23b-3p levels in cartilage and SCB osteocytes from human LS/HS tissues (n = 20). G The miR-23b-3p levels in cartilage and SCB osteocytes from mice with DMM surgery (n = 6). H The pre-miR-23b-3p levels in cartilage and SCB osteocytes from human LS/HS tissues (n = 20). I The pre-miR-23b-3p levels in cartilage and SCB osteocytes from mice with DMM surgery (n = 6). J Alcian blue staining. Scale bar in upper panel, 1 mm. Scale bar in lower panel, 2 mm. K Western blot analysis of COLII, ACAN, SOX9, MMP13 and ADAMTS5 protein expression in primary human and mouse chondrocytes transfected with nc or mimic (n = 3). L The schematic depicted the overexpression of miR-23b-3p in cartilage through intraarticular injection of rAAV5-miR-23b-3p-mcherry (rAAV5-miR-23b-3p) or negative control (rAAV5-nc) in 8-week-old male mice following DMM surgery (n = 8). M The Safranin O images of cartilage. Scale bar in upper panel, 200 μm. Scale bar in lower panel, 100 μm. Data are presented as the mean +/- SD. Data was analyzed by unpaired two-tailed Student’s t-test (D, E, G, I and K) and paired two-tailed Student’s t-test (F and H). Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40399261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.