ADAR Antibody (7H1U2)

Novus Biologicals | Catalog # NBP3-15383

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7H1U2 expressed in HEK293
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human ADAR (P55265). STQAWNQHSGVVRPDGHSQGAPNSDPSLEPEDRNSTSVSEDLLEPFIAVSAQAWNQHSGVVRPDSHSQGSPNSDPGLEPEDSNSTSALEDPLEFLDMAEIK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADAR Antibody (7H1U2)

Western Blot: ADAR Antibody (7H1U2) [NBP3-15383]

Western Blot: ADAR Antibody (7H1U2) [NBP3-15383]

Western Blot: ADAR Antibody (7H1U2) [NBP3-15383] - Analysis of extracts of various cell lines, using ADAR Rabbit mAb (NBP3-15383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383]

Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383]

Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383] - Mouse kidney using ADAR Rabbit mAb (NBP3-15383) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383]

Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383]

Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383] - Rat ovary using ADAR Rabbit mAb (NBP3-15383) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383]

Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383]

Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383] - Human colon using ADAR Rabbit mAb (NBP3-15383) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for ADAR Antibody (7H1U2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ADAR

ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Double-stranded RNA-specific adenosine deaminase

Alternate Names

ADAR1, DRADA, DSRAD, EC 3.5.4.37, G1P1, IFI-4, IFI4, K88DSRBP, p136

Gene Symbol

ADAR

Additional ADAR Products

Product Documents for ADAR Antibody (7H1U2)

Certificate of Analysis

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Product Specific Notices for ADAR Antibody (7H1U2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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