ADE2 Antibody (OTI1B4)

Novus Biologicals | Catalog # NBP2-02817

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1B4
View all ADE2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PAICS (NP_006443) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

46.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ADE2 Antibody (OTI1B4)

Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817]

Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817]

ADE2-Antibody-OTI1B4-Western-Blot-NBP2-02817-img0005.jpg
Immunocytochemistry/ Immunofluorescence: ADE2 Antibody (OTI1B4) [NBP2-02817]

Immunocytochemistry/ Immunofluorescence: ADE2 Antibody (OTI1B4) [NBP2-02817]

Immunocytochemistry/Immunofluorescence: ADE2 Antibody (OTI1B4) [NBP2-02817] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ADE2.
Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817]

Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817]

Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ADE2 antibody, and then analyzed by flow cytometry.
Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817]

Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817]

Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADE2.
Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817]

Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817]

Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817] - Analysis of Jurkat cells, using anti-ADE2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for ADE2 Antibody (OTI1B4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ADE2

This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Alternate Names

ADE2, AIR carboxylase, AIRCADE2H1, DKFZp781N1372, MGC1343, MGC5024, multifunctional protein ADE2, PAIS, phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazolesuccinocarboxamide synthetase, SAICAR synthetase

Entrez Gene IDs

10606 (Human)

Gene Symbol

PAICS

Additional ADE2 Products

Product Documents for ADE2 Antibody (OTI1B4)

Certificate of Analysis

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Product Specific Notices for ADE2 Antibody (OTI1B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ADE2 Antibody (OTI1B4)

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Protocols

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