Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70123

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4A1

Format

Azide and BSA Free
View all Adenylate Kinase 1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free

Western Blot: Adenylate Kinase 1 Antibody (OTI4A1)Azide and BSA Free [NBP2-70123]

Western Blot: Adenylate Kinase 1 Antibody (OTI4A1)Azide and BSA Free [NBP2-70123]

Western Blot: Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free [NBP2-70123] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adenylate Kinase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ade
Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free [NBP2-70123]

Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free [NBP2-70123]

Immunocytochemistry/Immunofluorescence: Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free [NBP2-70123] - Staining of HT29 cells using anti-Adenylate Kinase 1 mouse monoclonal antibody.
Flow Cytometry: Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free [NBP2-70123]

Flow Cytometry: Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free [NBP2-70123]

Flow Cytometry: Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free [NBP2-70123] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Adenylate Kinase 1 antibody, and then analyzed by flow cytometry.
Western Blot: Adenylate Kinase 1 Antibody (OTI4A1)Azide and BSA Free [NBP2-70123]

Western Blot: Adenylate Kinase 1 Antibody (OTI4A1)Azide and BSA Free [NBP2-70123]

Western Blot: Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free [NBP2-70123] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Adenylate Kinase 1 monoclonal antibody.

Applications for Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adenylate Kinase 1

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Long Name

Adenylate kinase isoenzyme 1

Alternate Names

AK 1, AK1, EC 2.7.4.3, EC 2.7.4.6, Myokinase

Gene Symbol

AK1

Additional Adenylate Kinase 1 Products

Product Documents for Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Adenylate Kinase 1 Antibody (OTI4A1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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