Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5C2

Format

Azide and BSA Free
View all ADI1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ADI1 (NP_060739) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse ADI1 Antibody (OTI5C2) - Azide and BSA Free (NBP2-03121) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ADI1 Antibody (OTI5C2) - Azide and BSA Free

Western Blot: ADI1 Antibody (OTI5C2)Azide and BSA Free [NBP2-71994]

Western Blot: ADI1 Antibody (OTI5C2)Azide and BSA Free [NBP2-71994]

Western Blot: ADI1 Antibody (OTI5C2) - Azide and BSA Free [NBP2-71994] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADI1.
Immunohistochemistry: ADI1 Antibody (OTI5C2) - Azide and BSA Free [NBP2-71994]

Immunohistochemistry: ADI1 Antibody (OTI5C2) - Azide and BSA Free [NBP2-71994]

Immunohistochemistry: ADI1 Antibody (OTI5C2) - Azide and BSA Free [NBP2-71994] - Staining of paraffin-embedded Human prostate tissue using anti-ADI1 mouse monoclonal antibody.
Flow Cytometry: ADI1 Antibody (OTI5C2) - Azide and BSA Free [NBP2-71994]

Flow Cytometry: ADI1 Antibody (OTI5C2) - Azide and BSA Free [NBP2-71994]

Flow Cytometry: ADI1 Antibody (OTI5C2) - Azide and BSA Free [NBP2-71994] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ADI1 antibody, and then analyzed by flow cytometry.

Applications for ADI1 Antibody (OTI5C2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: ADI1

ADI1 encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. A pseudogene that is located on chromosome 20 has been defined for this gene. [provided by RefSeq]

Alternate Names

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, Acireductone dioxygenase (Ni(2+)-requiring), acireductone dioxygenase 1, APL1, ARDsubmergence induced protein 2, EC 1.13.11.53, FLJ10913, HMFT1638, membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1, Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1, MTCBP1, MTCBP-1, Ni-ARD, SIPLMT1-MMP cytoplasmic tail-binding protein-1, Submergence-induced protein 2 homolog

Gene Symbol

ADI1

Additional ADI1 Products

Product Documents for ADI1 Antibody (OTI5C2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ADI1 Antibody (OTI5C2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ADI1 Antibody (OTI5C2) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review ADI1 Antibody (OTI5C2) - Azide and BSA Free and earn rewards!

Have you used ADI1 Antibody (OTI5C2) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies