AFAP Antibody (OTI2G9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72120

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2G9

Format

Azide and BSA Free
View all AFAP Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 100-386 of human AFAP1 (NP_940997) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AFAP Antibody (OTI2G9) - Azide and BSA Free

Western Blot: AFAP Antibody (OTI2G9)Azide and BSA Free [NBP2-72120]

Western Blot: AFAP Antibody (OTI2G9)Azide and BSA Free [NBP2-72120]

Western Blot: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP.
Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120]

Immunohistochemistry: AFAP Antibody (OTI2G9) - Azide and BSA Free [NBP2-72120] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for AFAP Antibody (OTI2G9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AFAP

AFAP, or Actin filament-associated protein 1, contains a 81 kDa and a 90 kDa isoform, and is involved in the manipulation of actin filaments to form network or bundle structures and the regulation of cell-matrix linkage. Disease research is currently being conducted with AFAP and its relation to familial adenomatous polyposis, adenocarcinoma, breast cancer, prostate adenocarcinoma, prostatitis, polyposis, prostate cancer, and carcinoma. This protein interacts with PRKCB, PRKCD, PRKCG, ACTC1, and PRKCZ during the processes of signal transduction regulation and mechanical stimulus response.

Long Name

Actin Filament-associated Protein, 110 kDa

Alternate Names

AFAP-110, AFAP1

Gene Symbol

AFAP1

Additional AFAP Products

Product Documents for AFAP Antibody (OTI2G9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AFAP Antibody (OTI2G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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