AFF4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89220

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AFF4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKS

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25252976).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AFF4 Antibody - BSA Free

Immunohistochemistry-Paraffin: AFF4 Antibody [NBP1-89220]

Immunohistochemistry-Paraffin: AFF4 Antibody [NBP1-89220]

Immunohistochemistry-Paraffin: AFF4 Antibody [NBP1-89220] - Staining of human hippocampus shows strong nuclear positivity in glial cells.

Applications for AFF4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AFF4

AFF4, or AF4/FMR2 family member 4, contains a 127 kDa, 98 kDa, and 39 kDa isoform, and is involved in the process of improving the catalytic rate of RNA polymerase II transcription. Disease research is currently being conducted with AFF4 on its relation to acute lymphoblastic leukemia, as well as other types of leukemia. This protein interacts with SIAH1, CCNT1, MLLT3, MLLT1, and MED10 during the regulation of DNA-dependent transcription and the transcription of RNA polymerase II.

Alternate Names

AF4/FMR2 family, member 4, AF5Q31AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, Major CDK9 elongation factor-associated protein, MCEFALL1 fused gene from 5q31, MGC75036, Protein AF-5q31

Entrez Gene IDs

27125 (Human)

Gene Symbol

AFF4

UniProt

Q9UHB7 (Human)

Additional AFF4 Products

Product Documents for AFF4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for AFF4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AFF4 Antibody - BSA Free

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Protocols

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FAQs

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