AGER Antibody

Novus Biologicals | Catalog # NBP3-13484

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Amphibian, Squirrel

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RAGE/AGER Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat AGER.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for AGER Antibody

Western Blot: AGER Antibody [NBP3-13484]

Western Blot: AGER Antibody [NBP3-13484]

Western Blot: AGER Antibody [NBP3-13484] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AGER antibody (NBP3-13484) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484]

Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484]

Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484] - IHC-P analysis of normal mouse lymph node tissue with (ledt) or without (right) AGER antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Western Blot: AGER Antibody [NBP3-13484]

Western Blot: AGER Antibody [NBP3-13484]

Western Blot: AGER Antibody [NBP3-13484] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with AGER antibody (NBP3-13484) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484]

Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484]

Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484] - AGER antibody detects AGER protein in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. AGER antibody (NBP3-13484) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484]

Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484]

Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484] - IHC-P analysis of normal mouse heart tissue with (ledt) or without (right) AGER antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484]

Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484]

Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484] - IHC-P analysis of normal mouse kidney tissue with (ledt) or without (right) AGER antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Applications for AGER Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1-2 ug/ml

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAGE/AGER

AGER encodes a member of the immunoglobulin superfamily of cell surface molecules. It is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products. The gene lies within the major histocompatibility complex (MHC) class III region on chromosome 6. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Long Name

Receptor for Advanced Glycation End Products

Alternate Names

AGER, SCARJ1

Gene Symbol

AGER

Additional RAGE/AGER Products

Product Documents for AGER Antibody

Certificate of Analysis

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Product Specific Notices for AGER Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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