AGO1/2 Antibody

Novus Biologicals | Catalog # NBP3-18654

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all AGO1/2 Primary Antibodies »

Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against human AGO1/2 (eukaryotic translation initiation factor 2C1 and 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of both EIF2C1 and EIF2C2.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for AGO1/2 Antibody

Western Blot: AGO1/2 Antibody [NBP3-18654]

Western Blot: AGO1/2 Antibody [NBP3-18654]

Western Blot: AGO1/2 Antibody [NBP3-18654] - Figure 2. Western blot analysis using the antibody directed against AGO1/2 Whole cell extracts of U2OS cells (40 ug) were analysed by Western blot using the antibody against AGO1/2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 97 kDa); the marker (in kDa) is shown on the left.
ELISA: AGO1/2 Antibody [NBP3-18654]

ELISA: AGO1/2 Antibody [NBP3-18654]

ELISA: AGO1/2 Antibody [NBP3-18654] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human AGO1/2. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,500.

Applications for AGO1/2 Antibody

Application
Recommended Usage

ELISA

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: AGO1/2

Alternate Names

argonaute 1, RISC catalytic component, argonaute 2, RISC catalytic component, argonaute RISC catalytic component 1, argonaute1, cancer susceptibility candidate 7 (non-protein coding), CASC7, EIF2C, eIF2C 1, eIF-2C 1, EIF2C1, EIF2C2, eukaryotic translation initiation factor 2C, 1, eukaryotic translation initiation factor 2C, 2, GERP95, Golgi Endoplasmic Reticulum protein 95 kDa, hAgo1, LESKRES, LINC00980, long intergenic non-protein coding RNA 980, PAZ Piwi domain protein, PPD, protein argonaute-1, protein argonaute-2, Protein slicer, Putative RNA-binding protein Q99, Q10, Q99

Gene Symbol

AGO1

UniProt

Q9UL18 (Human)

Additional AGO1/2 Products

Product Documents for AGO1/2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for AGO1/2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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