Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # PCRP-CREB3L4-1A3

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fragment (aa1-298) of human AIBZIP protein

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Description

Antibody without azide - store at -20 to -80C.

Scientific Data Images for AIBZIP Antibody (PCRP-CREB3L4-1A3) - Azide and BSA Free

Immunohistochemistry-Paraffin: AIBZIP Antibody (PCRP-CREB3L4-1A3) - Azide and BSA Free [NBP3-24138] -

Formalin-fixed, paraffin-embedded human prostate cancer stained with AIBZIP antibody (PCRP-CREB3L4-1A3) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Flow Cytometry: AIBZIP Antibody (PCRP-CREB3L4-1A3) - Azide and BSA Free [NBP3-24138] -

Flow Cytometric Analysis of PFA-fixed HeLa cells. AIBZIP antibody (PCRP-CREB3L4-1A3) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: AIBZIP Antibody (PCRP-CREB3L4-1A3) - Azide and BSA Free [NBP3-24138] -

Analysis of Protein Array containing more than 19,000 full-length human proteins using AIBZIP antibody (PCRP-CREB3L4-1A3) - Azide and BSA Free

Applications for AIBZIP Antibody (PCRP-CREB3L4-1A3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: AIBZIP

Androgen-induced basic leucine zipper (AIBZIP)is a transcription factor. AIbZIP is a 395 aa protein with homology to cyclic AMP-responsive element binding protein/activating transcription factor. It contains an NH(2)-terminal activation domain, a central bZIP domain, and a COOH-terminal transmembrane domain. Immunoreactive AIbZIP protein has been detected in the cytoplasm of prostatic luminal epithelial cells; full-length AIbZIP-GFP fusion proteins are localized in the cytoplasm of LNCaP cells, while a truncated form of AIbZIP lacking the putative transmembrane domain was exclusively nuclear. AIbZIP is expressed at higher levels in cancerous prostate cells compared with noncancerous prostate cells.

Alternate Names

AIbZIP, AIBZIPcAMP-responsive element-binding protein 4, Androgen-induced basic leucine zipper protein, ATCE1JAL, Attaching to CRE-like 1, cAMP responsive element binding protein 1, cAMP responsive element binding protein 3-like 4, CREB3, CREB4CREB-4, cyclic AMP-responsive element-binding protein 3-like protein 4, Cyclic AMP-responsive element-binding protein 4, hJALcAMP-responsive element-binding protein 3-like protein 4, tisp40, Transcript induced in spermiogenesis protein 40

Gene Symbol

CREB3L4

Additional AIBZIP Products

Product Documents for AIBZIP Antibody (PCRP-CREB3L4-1A3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AIBZIP Antibody (PCRP-CREB3L4-1A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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