Alas1 Antibody

Novus Biologicals | Catalog # NB100-56415

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Predicted:

Canine (94%), Chicken (94%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Alas1 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 480-530 of human ALAS-1 were used as the immunogen.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous in human, chimp and beluga whale; 94% in dog and chicken; 88% in mouse, rat and lungfish; 83% in bovine, xenopus, pufferfish; 77% in coelacanth. Rat reactivity reported in scientific literature (PMID: 19467246).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alas1 Antibody

Western Blot: Alas1 Antibody [NB100-56415]

Western Blot: Alas1 Antibody [NB100-56415]

Western Blot: Alas1 Antibody [NB100-56415] - analysis of HepG2 cell lysate using ALAS1 antibody at1 ug/ml.
Immunohistochemistry-Paraffin: Alas1 Antibody [NB100-56415]

Immunohistochemistry-Paraffin: Alas1 Antibody [NB100-56415]

Immunohistochemistry-Paraffin: Alas1 Antibody [NB100-56415] - Staining of human skin at 5 ug/ml. Heat induced antigen retrieval in pH 6.0 citrate buffer was used for unmasking. A biotinylated secondary antibody followed by alkaline phosphatase-streptavidin was and chromogen was used for detection.
Simple Western: Alas1 Antibody [NB100-56415]

Simple Western: Alas1 Antibody [NB100-56415]

Simple Western: Alas1 Antibody [NB100-56415] - Simple Western lane view shows a specific band for Alas1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Alas1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:50

Western Blot

1-3 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 65 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alas1

Official Gene Symbol: ALAS1 Gene ID: 211(human) Gene Map Locus: 3p21.1(human) ALAS-1 (EC 2.3.1.37) is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.

Alternate Names

5-aminolevulinic acid synthase 1, ALAS, ALAS3, ALASH, ALAS-H, aminolevulinate, delta-, synthase 1, Delta-ALA synthase 1,5-aminolevulinate synthase, nonspecific, mitochondrial, Delta-aminolevulinate synthase 1, EC 2.3.1, EC 2.3.1.37, MIG4, migration-inducing protein 4

Entrez Gene IDs

211 (Human)

Gene Symbol

ALAS1

UniProt

NP_065584 (Human)

Additional Alas1 Products

Product Documents for Alas1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Alas1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Alas1 Antibody

There are currently no reviews for this product. Be the first to review Alas1 Antibody and earn rewards!

Have you used Alas1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies