Albumin Antibody - BSA Free

Novus Biologicals | Catalog # NB300-336

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Format

BSA Free
View all Albumin Primary Antibodies »

Product Specifications

Immunogen

Recombinant serum albumin (GI # 113576) sequence 1-609

Reactivity Notes

Cross-reacts with Human, Mouse, Rat, Cow, Dog and Pig. Not yet tested in other species.

Localization

Secreted

Specificity

Albumin

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Scientific Data Images for Albumin Antibody - BSA Free

ELISA: Albumin Antibody - BSA Free [NB300-336]

ELISA: Albumin Antibody - BSA Free [NB300-336]

ELISA: Albumin Antibody [NB300-336] - Direct ELISA Test. Human serum albumin protein as test antigen. Affi-pure anti-HSA IgY as primary antibody (0.1 ug/ml), and Rabbit anti-IgY-HRP as 2nd antibody. ( anti-ALB )

Applications for Albumin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:200-1:500
Application Notes
This antibody can be used in ELISA and Western Blot. In Western Blot a 69.4 kDa band is seen. Recommended Starting Dilutions*: ELISA: 0.1-0.5 ug/ml WB: 1/1000 Not tested in other applications. *Optimal dilutions should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Albumin

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Alternate Names

ALB

Entrez Gene IDs

213 (Human); 11657 (Mouse); 24186 (Rat)

Gene Symbol

ALB

Additional Albumin Products

Product Documents for Albumin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Albumin Antibody - BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Acute Phase Proteins

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