Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Novus Biologicals | Catalog # NB100-787

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EVKTVTVKISQKNS corresponding to C-Terminus according to NP_000680.2.

Marker

Astrocyte Marker

Specificity

This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design, and with ALDH2 (GeneID 217) with two residues difference from this design.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] - Staining of human (A), mouse (B) and rat (C) liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] -

Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

detection limit 1:1000

Western Blot

0.1-0.3 ug/ml
Application Notes
WB: Approx 55 kDa band observed in human, mouse and rat liver lysates (calculated MW of 54.9 kDa according to NP_000680.2). IHC: successful usage on mouse Peyer's patches is reported in scientific literature (PMID: 15485630).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1

A protease inhibitor that inhibits fibrin clot formation.

Alternate Names

ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1

Entrez Gene IDs

216 (Human); 11668 (Mouse); 24188 (Rat)

Gene Symbol

ALDH1A1

UniProt

NP_000680 (Human)

Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products

Product Documents for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

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Protocols

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