ALDH Detection Reagent - BAAA-DA
Tocris Bioscience | Catalog # 7556
Key Product Details
Description
Wavelength
Product Description
Key information: ALDH Detection Reagent - BAAA-DA is a fluorescent substrate of aldehyde dehydrogenase (ALDH).
Used for: can be used in flow cytometry to sort cancer stem cells with high ALDH levels, and to isolate human hematopoietic progenitor cells. Can be used with efflux inhibitors such as Verapamil (Cat. No. 0654), Probenecid (Cat. No. 4107) or 2-deoxy-D-glucose (Cat. No. 4515) to block multi-drug resistance efflux.
Application: flow cytometry, fluorescent-activated cell sorting (FACS).
Properties and Photophysical Data: BAAA-DA is converted under acidic conditions to BAAA. BAAA, cell-permeant, is converted by intracellular ALDH into carboxylate ion amino acetate (BAA) and used to identify cells with high ALDH activity. Excitation and emission maxima (λ) are 505 nm and 512 nm, respectively; extinction coefficient = 74,595 M-1cm-1.
Optical Data
| λabs | 505 nm |
| λem | 512 nm |
| Extinction Coefficient (ε) | 74595 M-1cm-1 |
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Product Specifications for ALDH Detection Reagent - BAAA-DA
Molecular Weight
Formula
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Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.07 | 10 |
Preparing Stock Solutions for ALDH Detection Reagent - BAAA-DA
The following data is based on the product molecular weight 407.27.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 24.55 mL | 122.77 mL | 245.54 mL |
| 0.5 mM | 4.91 mL | 24.55 mL | 49.11 mL |
| 1 mM | 2.46 mL | 12.28 mL | 24.55 mL |
| 5 mM | 0.49 mL | 2.46 mL | 4.91 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Storms Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity. Proc.Natl.Acad.Sci.U.S.A. 1999 PMID: 10430905
Product Documents for ALDH Detection Reagent - BAAA-DA
Certificate of Analysis
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Product Specific Notices for ALDH Detection Reagent - BAAA-DA
For research use only
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