Verapamil hydrochloride

Tocris Bioscience | Catalog # 0654

CaV1.x blocker
Tocris Bioscience

Key Product Details

Description

CaV1.x blocker
View all Cav1.x Channel Blockers »

Product Description

Verapamil hydrochloride is an L-type calcium channel blocker, anti-arrythmic, vasodilator and adrenergic antagonist. Induces autophagy by modulating cellular metabolism. Verapamil is a P-glycoprotein inhibitor and can reverse resistance to glucocorticoids and cancer treatments. Verapamil has been identified as targeting human host proteins that interact with SARS-CoV-2.

Scientific Data Images for Verapamil hydrochloride

Pluripotent stem cell-derived cardiomyocytes secrete Pro-BNP in response to small molecule-induction of cardiac hypertrophy.

Pluripotent stem cell-derived cardiomyocytes secrete Pro-BNP in response to small molecule-induction of cardiac hypertrophy.

Cardiomyocytes were treated with the vasoconstrictor, ET-1 (Endothelin-1; Catalog # 1160), or one of two L-type calcium channel inhibitors, Doxorubicin (Catalog # 2252) or Verapamil (Catalog # 0654). Levels of the cardiac hypertrophy marker, Pro-Brain Natriuretic Peptide (Pro-BNP), were detected via Luminex® bead-based multiplex assay (Catalog # LXSAH). A substantial increase in Pro-BNP was detected in the medium of cardiomyocytes treated with ET-1, a known inducer of cardiac hypertrophy. Conversely, Doxorubicin and Verapamil decreased Pro-BNP secretion from cardiomyocytes.

Verapamil affects cardiomyocyte contraction rates

Verapamil affects cardiomyocyte contraction rates.

The effect of small molecules on cardiomyocyte contraction rate was assessed by monitoring intracellular calcium fluctuations, where an increase in calcium coincides with cardiomyocyte contraction. Cardiomyocytes were loaded with the fluorescent calcium indicator, Fluo-4, prior to small molecule treatment. Individual wells were first monitored for baseline rates of calcium fluctuation represented as beats per minute (bpm). Verapamil (10uM or 30uM, Catalog # 0654), an L-type calcium channel blocker, decreased the rate of calcium fluctuations in cardiomyocytes in a dose-dependent manner.

Product Specifications for Verapamil hydrochloride

Molecular Weight

491.07

Formula

C27H38N2O4.HCl

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

α-[3-[[2-(3,4-Dimethoxyphenyl)ethyl]methylamino]propyl]-3,4-dimethoxy-α-(1-methylethyl)benzeneacetonitrile hydrochloride

CAS Number

152-11-4

PubChem ID

62969

InChI Key

DOQPXTMNIUCOSY-UHFFFAOYSA-N

SMILES

Cl.COC1=CC=C(C=C1OC)C(CCCN(C)CCC1=CC(OC)=C(OC)C=C1)(C#N)C(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 24.55 50
DMSO 24.55 50

Preparing Stock Solutions for Verapamil hydrochloride

The following data is based on the product molecular weight 491.07.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 4.07 mL 20.36 mL 40.73 mL
2.5 mM 0.81 mL 4.07 mL 8.15 mL
5 mM 0.41 mL 2.04 mL 4.07 mL
25 mM 0.08 mL 0.41 mL 0.81 mL

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Product Documents for Verapamil hydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Verapamil hydrochloride

For research use only

Related Research Areas

Ion Channels and Regulators

Citations for Verapamil hydrochloride

Customer Reviews for Verapamil hydrochloride (1)

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  • Inhibits calcium influx
    Name: Mohammad Alqudah
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 10/19/2022
    Used to inhibit calcium influx in an organ bath at 10 uM. Was dissolved in water. Good inhibition
    Verapamil hydrochloride 0654

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