ALDH2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31749

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Predicted:

Bovine (93%), Porcine (92%), Rat (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ALDH2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (83%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH2 Antibody - BSA Free

Western Blot: ALDH2 Antibody [NBP1-31749]

Western Blot: ALDH2 Antibody [NBP1-31749]

Western Blot: ALDH2 Antibody [NBP1-31749] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody [NBP1-31749]

Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody [NBP1-31749]

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody [NBP1-31749] - Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min. Green: ALDH2 protein stained by ALDH2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Western Blot: ALDH2 Antibody [NBP1-31749]

Western Blot: ALDH2 Antibody [NBP1-31749]

Western Blot: ALDH2 Antibody [NBP1-31749] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE; antibody diluted at 1:10000.
ALDH2 Antibody

Western Blot: ALDH2 Antibody [NBP1-31749] -

Western Blot: ALDH2 Antibody [NBP1-31749] - Sample (30 ug of whole cell lysate)
A: whole zebrafish
7.5% SDS PAGE
NBP1-31749 diluted at 1:1000
ALDH2 Antibody

Immunohistochemistry: ALDH2 Antibody [NBP1-31749] -

Immunohistochemistry: ALDH2 Antibody [NBP1-31749] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ALDH2 antibody [N1C1] (NBP1-31749) at 1:300 dilution.
ALDH2 Antibody

Immunohistochemistry: ALDH2 Antibody [NBP1-31749] -

Immunohistochemistry: ALDH2 Antibody [NBP1-31749] - ALDH2 antibody [N1C1] detects ALDH2 protein at mitochondria on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
ALDH2 antibody [N1C1] (NBP1-31749) dilution: 1:200.

Applications for ALDH2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ALDH2

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix.

Long Name

Aldehyde Dehydrogenase 2 Family Member

Alternate Names

ALDHI, ALDM

Gene Symbol

ALDH2

Additional ALDH2 Products

Product Documents for ALDH2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ALDH2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Redox Enzymes

Citations for ALDH2 Antibody - BSA Free

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Protocols

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