ALDH4A1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89149

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KSLLNKAIEAALAARKEWDLKPIADRAQIFLKAADMLSGPRRAEILAKTMVGQGKTVIQAEIDAAAELIDFFRFNAKYAVELEGQQPISVPPSTNSTVYRGLE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH4A1 Antibody - BSA Free

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149]

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149]

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] - Analysis in human kidney and lymph node tissues using NBP1-89149 antibody. Corresponding ALDH4A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149]

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149]

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] - Staining of human kidney shows high expression.
ALDH4A1 Antibody

Western Blot: ALDH4A1 Antibody [NBP1-89149] -

Western Blot: ALDH4A1 Antibody [NBP1-89149] -Analysis in human kidney tissue.
ALDH4A1 Antibody

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] -

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
ALDH4A1 Antibody

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] -

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.
ALDH4A1 Antibody

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] -

Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] - Staining of human lymph node shows no positivity in lymphoid cells as expected.

Applications for ALDH4A1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH4A1

ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Two transcript variants encoding the same protein have been identified for this gene.

Alternate Names

aldehyde dehydrogenase 4 family, member A1, Aldehyde dehydrogenase family 4 member A1, ALDH4DKFZp779M035, delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial, EC 1.5.1.12, mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase, P5C dehydrogenase, P5CD, P5CDH

Entrez Gene IDs

8659 (Human)

Gene Symbol

ALDH4A1

UniProt

Additional ALDH4A1 Products

Product Documents for ALDH4A1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ALDH4A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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