Aldo-keto Reductase 1C1/AKR1C1 Antibody (5-F10-R)

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody (5-F10-R) [NBP3-32014]

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody (5-F10-R) [NBP3-32014] - Western blot analysis of Aldo-keto Reductase 1C1/AKR1C1 on different lysates with Mouse anti-Aldo-keto Reductase 1C1/AKR1C1 antibody (NBP3-32014) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: U-87 MG cell lysate (20 ug/Lane)
Lane 3: HeLa cell lysate (20 ug/Lane)
Lane 4: A431 cell lysate (20 ug/Lane)
Lane 5: Human liver tissue lysate (40 ug/Lane)

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32014) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1C1/AKR1C1 Antibody (5-F10-R) [NBP3-32014]

Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1C1/AKR1C1 Antibody (5-F10-R) [NBP3-32014] - Immunocytochemistry analysis of HeLa cells labeling Aldo-keto Reductase 1C1/AKR1C1 with Mouse anti-Aldo-keto Reductase 1C1/AKR1C1 antibody (NBP3-32014) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Aldo-keto Reductase 1C1/AKR1C1 antibody (NBP3-32014) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.