Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25355

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Drosophila

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1268 expressed in HEK293

Format

Azide and BSA Free
View all Alkaline Phosphatase, Intestinal Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase, Intestinal. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%), Bovine (85%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free (NBP3-25355) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images

Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] -

Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] -

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] -

Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] -

Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] -

Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] -

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Intestinal

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Long Name

Alkaline Phosphatase, Intestinal Type

Alternate Names

alkaline phosphatase, intestinal, Alkaline phosphomonoesterase, ALPI, EC 3.1.3.1, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, intestinal-type alkaline phosphatase, Kasahara isozyme

Gene Symbol

ALPI

Additional Alkaline Phosphatase, Intestinal Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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