alpha-Actinin 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85791

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all alpha Actinin 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKV

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261). Canine reactivity per customer review.

Marker

Z-disk Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha-Actinin 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-85791]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-85791]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-85791] - Staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Western Blot: alpha-Actinin 1 Antibody [NBP1-85791]

Western Blot: alpha-Actinin 1 Antibody [NBP1-85791]

Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
alpha-Actinin 1 Antibody - BSA Free Western Blot: alpha-Actinin 1 Antibody - BSA Free [NBP1-85791]

Western Blot: alpha-Actinin 1 Antibody - BSA Free [NBP1-85791]

Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
alpha-Actinin 1 Antibody - BSA Free Western Blot: alpha-Actinin 1 Antibody - BSA Free [NBP1-85791]

Western Blot: alpha-Actinin 1 Antibody - BSA Free [NBP1-85791]

Analysis in human cell lines A-431 and HEK293 using Anti-ACTN1 antibody. Corresponding ACTN1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Applications for alpha-Actinin 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 2 reviews rated 3 using NBP1-85791 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha Actinin 1

Alpha Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle alpha actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle alpha actinin has been detected in dense bodies and plaques characteristic of the tissue. Immunofluorescent labeling of a large variety of cells using antibody to alpha actinin reveals extensive association of the proteins with actin containing stress fibers and in particular with their membrane bound termini.

Long Name

Actinin alpha 1

Alternate Names

ACTN1, alpha-Actinin 1, alphaActinin 1, BDPLT15

Gene Symbol

ACTN1

Additional alpha Actinin 1 Products

Product Documents for alpha-Actinin 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for alpha-Actinin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Actinin 1 Antibody - BSA Free

Customer Reviews for alpha-Actinin 1 Antibody - BSA Free (2)

3 out of 5
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Verified Customer | Posted 08/28/2014
    Dog Diaphragm, 25 ug total protein loaded
    alpha-Actinin 1 Antibody - BSA Free NBP1-85791
  • Name: Anonymous
    Application: Western Blot
    Verified Customer | Posted 08/28/2014
    Mouse Triceps, 25ug total protein
    alpha-Actinin 1 Antibody - BSA Free NBP1-85791

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Protocols

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FAQs for alpha-Actinin 1 Antibody - BSA Free

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  • Q: Is this item restricted under "Cartagena Law"?

    A: To our knowledge this item is not restricted under the Cartagena Protocol, but this antibody is RUO. We have not tested or certified this product for use in vivo or for diagnostic use. Any use of this kind is undertaken by the end user at their own risk.

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