Alpha Actinin 4 Antibody (5Y3R6)

Name: Alpha Actinin 4 Antibody (5Y3R6)
Brand: Novus Biologicals
SKU: NBP3-16176
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16176

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5Y3R6 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Alpha Actinin 4 (O43707). MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alpha Actinin 4 Antibody (5Y3R6)

Western Blot: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176]

Western Blot: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176] - Western blot analysis of extracts of various cell lines, using Alpha Actinin 4 Rabbit mAb (NBP3-16176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunoprecipitation: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176]

Immunoprecipitation: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Alpha Actinin 4 antibody (NBP3-16176). Western blot was performed from the immunoprecipitate using Alpha Actinin 4 antibody (NBP3-16176) at a dilition of 1:1000.

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176] -

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176] - Confocal imaging of U-2 OS cells using Alpha Actinin 4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for Alpha Actinin 4 Antibody (5Y3R6)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Alpha Actinin 4

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Alternate Names

actinin alpha4 isoform, actinin, alpha 4, ACTININ-4, alpha-actinin-4, DKFZp686K23158, F-actin cross-linking protein, focal segmental glomerulosclerosis 1, FSGS, FSGS1, Non-muscle alpha-actinin 4

Gene Symbol

ACTN4

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Product Documents for Alpha Actinin 4 Antibody (5Y3R6)

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Certificate of Analysis

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Product Specific Notices for Alpha Actinin 4 Antibody (5Y3R6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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