alpha Adducin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15359

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Predicted:

Bovine (99%), Chicken (98%), Rat (98%), Rhesus Macaque (100%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all alpha Adducin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Adducin. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (93%), Zebrafish (89%).

Localization

Cytoplasm; cytoskeleton; Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha Adducin Antibody - BSA Free

Western Blot: alpha Adducin Antibody [NBP2-15359]

Western Blot: alpha Adducin Antibody [NBP2-15359]

Western Blot: alpha Adducin Antibody [NBP2-15359] - A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE alpha Adducin antibody [N1N2], N-term dilution: 1:1000
Immunohistochemistry-Paraffin: alpha Adducin Antibody [NBP2-15359]

Immunohistochemistry-Paraffin: alpha Adducin Antibody [NBP2-15359]

Immunohistochemistry-Paraffin: alpha Adducin Antibody [NBP2-15359] - Paraffin-embedded SG xenograft, using antibody at 1:100 dilution.
Western Blot: alpha Adducin Antibody [NBP2-15359]

Western Blot: alpha Adducin Antibody [NBP2-15359]

Western Blot: alpha Adducin Antibody [NBP2-15359] - Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE; antibody diluted at 1:1000.
alpha Adducin Antibody

Immunohistochemistry: alpha Adducin Antibody [NBP2-15359] -

Immunohistochemistry: alpha Adducin Antibody [NBP2-15359] - alpha Adducin antibody [N1N2], N-term detects alpha Adducin protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
alpha Adducin antibody [N1N2], N-term (NBP2-15359) dilution: 1:200.

Applications for alpha Adducin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha Adducin

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq]

Alternate Names

ADDA, adducin 1 (alpha), alpha-adducin, erythrocyte adducin alpha subunit, Erythrocyte adducin subunit alpha, MGC3339, MGC44427

Gene Symbol

ADD1

UniProt

P35611 (Mouse)

Additional alpha Adducin Products

Product Documents for alpha Adducin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for alpha Adducin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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