alpha-Galactosidase A/GLA Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16576

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all alpha-Galactosidase A/GLA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha-Galactosidase A/GLA. The exact sequence is proprietary.

Reactivity Notes

Pig (84%), Bovine (80%).

Localization

Lysosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Galactosidase A/GLA Antibody - BSA Free

Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP2-16576]

Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP2-16576]

Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576]

Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576]

Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Rat tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunoprecipitation: alpha-Galactosidase A/GLA Antibody [NBP2-16576]

Immunoprecipitation: alpha-Galactosidase A/GLA Antibody [NBP2-16576]

Immunoprecipitation: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Immunoprecipitation of GLA protein from 293T whole cell extracts using 5 ug of Galactosidase alpha antibody [N1C2]. Western blot analysis was performed using Galactosidase alpha antibody [N1C2]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
alpha-Galactosidase A/GLA Antibody - BSA Free

Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] -

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] (NBP2-16576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for alpha-Galactosidase A/GLA Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha-Galactosidase A/GLA

This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]

Alternate Names

Agalsidase alpha, GALA, Melibiase

Gene Symbol

GLA

UniProt

P06280

Additional alpha-Galactosidase A/GLA Products

Product Documents for alpha-Galactosidase A/GLA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for alpha-Galactosidase A/GLA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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