Alpha-mannosidase II Antibody (PSH02-67)

Western Blot: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565]

Western Blot: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Western blot analysis of Alpha-mannosidase II on different lysates with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: Huh7 cell lysate
Lane 3: JAR cell lysate
Lane 4:U-87 MG cell lysate
Lane 5: HUVEC cell lysate
Lane 6: A375 cell lysate
Lane 7: MDA-MB-468 cell lysate
Lane 8: NCI-H226 cell lysate
Lane 9: Human liver tissue lysate
Lane 10: human lung tissue lysate
Lane 11: human kidney tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 131 kDa
Observed band size: 131-151 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565]

Immunohistochemistry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32565) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565]

Flow Cytometry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Flow cytometric analysis of U-87 MG cells labeling Alpha-mannosidase II.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32565, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).