alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31555

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha-N-acetylglucosaminidase/NAGLU. The exact sequence is proprietary.

Reactivity Notes

Bovine (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555]

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555]

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunocytochemistry/ Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555]

Immunocytochemistry/ Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555]

Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555]

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555]

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555]

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555]

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Rat duodenum. NAGLU antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555]

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555]

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse brain. NAGLU antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] -

alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha-N-acetylglucosaminidase/NAGLU protein stained by alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:250.
Blue: Hoechst 33342 staining.
alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] -

alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488).
Blue: Nuclear staining with Hoechst 33342.
^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

Immunohistochemistry-Frozen: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] -

alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: alpha-N-acetylglucosaminidase/NAGLU stained by alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Applications for alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha-N-acetylglucosaminidase/NAGLU

This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq]

Alternate Names

alphaNacetylglucosaminidase, NAG, NAGLU, UFHSD1

Entrez Gene IDs

4669 (Human)

Gene Symbol

NAGLU

UniProt

Additional alpha-N-acetylglucosaminidase/NAGLU Products

Product Documents for alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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