Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP2-66423

Novus Biologicals
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.38 ng/mL

Assay Range

0.63-40 ng/mL
View all alpha-Smooth Muscle Actin ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

This kit recognizes Human ACT(alpha)2 in samples. No significant cross-reactivity or interference between Human ACT(alpha)2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 9.77%

Inter-Assay Precision (Precision between assays) CV% 7.69%

Recovery for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)

Recovery

86-114%

Linearity

Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)

Scientific Data Images for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)

Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)

ELISA: Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence) [NBP2-66423] -

ELISA: Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence) [NBP2-66423] - Standard Curve Reference

Kit Contents for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)

  • Concentrated Biotinylated Detection Antibody (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • Detection Antibody Diluent
  • HRP Conjugate Diluent
  • Micro CLIA Plate(Dismountable)
  • Plate Sealer
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2.El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G.,... Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M.,... Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customer Reviews for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)

There are currently no reviews for this product. Be the first to review Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence) and earn rewards!

Have you used Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?

    A: We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for ELISA Kits