Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # CRYAB/7917

Format

Azide and BSA Free
View all AlphaB Crystallin/CRYAB Primary Antibodies »

Product Specifications

Immunogen

Recombinant human full-length AlphaB Crystallin/CRYAB protein

Localization

Cytoplasm, translocates to nucleus during heat shock and resides in nuclear splicing speckles.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

Positive Controls: Huma heart, kidney or brain.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for AlphaB Crystallin/CRYAB Antibody (CRYAB/7917) - Azide and BSA Free

AlphaB Crystallin/CRYAB Antibody (CRYAB/7917) - Azide and BSA Free

AlphaB Crystallin/CRYAB Antibody (CRYAB/7917)

Formalin-fixed, paraffin-embedded human heart stained with AlphaB Crystallin/CRYAB antibody (CRYAB/7917). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for AlphaB Crystallin/CRYAB Antibody (CRYAB/7917) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: AlphaB Crystallin/CRYAB

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Gene Symbol

CRYAB

Additional AlphaB Crystallin/CRYAB Products

Product Documents for AlphaB Crystallin/CRYAB Antibody (CRYAB/7917) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AlphaB Crystallin/CRYAB Antibody (CRYAB/7917) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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