![Western Blot: AMBRA1 Antibody [NBP2-24723]](https://resources.rndsystems.com/images/products/AMBRA1-Antibody-Western-Blot-NBP2-24723-img0001.jpg "Western Blot: AMBRA1 Antibody [NBP2-24723]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Chicken
Predicted:
Canine (100%), Chimpanzee (100%), Monkey (100%), Opossum (100%), Porcine (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
A portion of amino acids 1-50 of human Ambra1 was used as the immunogen for the antibody.
Reactivity Notes
The amino acid sequence used as immunogen for the antibody is 100% homologous in Macaques.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit AMBRA1 Antibody (NBP2-24723) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for AMBRA1 Antibody
Western Blot: AMBRA1 Antibody [NBP2-24723]
Western Blot: AMBRA1 Antibody [NBP2-24723] - analysis of Ambra1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using Ambra1 antibody at 1 ug/ml.![Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-24723]](https://resources.rndsystems.com/images/products/AMBRA1-Antibody-Immunohistochemistry-Paraffin-NBP2-24723-img0002.jpg "Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-24723]")
Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-24723]
Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-24723] - Formalin-fixed, paraffin-embedded human small intestine stained with Ambra1 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Applications for AMBRA1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
0.5-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AMBRA1
Alternate Names
activating molecule in BECN1-regulated autophagy protein 1, autophagy/beclin-1 regulator 1, DCAF3, DDB1 and CUL4 associated factor 3, FLJ20294, KIAA1736activating molecule in beclin-1-regulated autophagy, MGC33725, WD repeat domain 94, WDR94
Gene Symbol
AMBRA1
UniProt
Additional AMBRA1 Products
Product Documents for AMBRA1 Antibody
Product Specific Notices for AMBRA1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for AMBRA1 Antibody
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Q: I need to know the peptide region used for preparation of the antibody NBP2-24723 to verify specificity with my model organism (zebrafish).
A: Unfortunately, I am not able to share the exact sequence used to generate NBP2-24723 as that information is proprietary; however, I am happy to give you a range. The immunogen for this antibody lies within aa 15-45 of the human sequence.
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