Aminopeptidase O/ONPEP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89066

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Aminopeptidase O/ONPEP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ECSSWYYYVTMPMPASTFTIAVGCWTEMKMETWSSNDLATERPFSPSEANFRHVGVCSHMEYPCRFQNASATTQEIIPHRVFAPVCLTGACQETLLRLIPPCLSAAHSVLGAHPFSRLDVLIVPANFPSLGMA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aminopeptidase O/ONPEP Antibody - BSA Free

Western Blot: Aminopeptidase O/ONPEP Antibody [NBP1-89066]

Western Blot: Aminopeptidase O/ONPEP Antibody [NBP1-89066]

Western Blot: Aminopeptidase O/ONPEP Antibody [NBP1-89066] - Analysis in control (vector only transfected HEK293T lysate) and C9orf3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Aminopeptidase O/ONPEP Antibody [NBP1-89066]

Immunohistochemistry-Paraffin: Aminopeptidase O/ONPEP Antibody [NBP1-89066]

Immunohistochemistry-Paraffin: Aminopeptidase O/ONPEP Antibody [NBP1-89066] - Staining of human kidney shows strong cytoplasmic positivity in tubule cells.

Applications for Aminopeptidase O/ONPEP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aminopeptidase O/ONPEP

Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or proteinsubstrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensinpathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing ofbioactive peptides in tissues such as testis and heart

Alternate Names

APO, C90RF3, C9orf3, ONPEP

Entrez Gene IDs

84909 (Human)

Gene Symbol

AOPEP

UniProt

Q8N6M6

Additional Aminopeptidase O/ONPEP Products

Product Documents for Aminopeptidase O/ONPEP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Aminopeptidase O/ONPEP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

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Protocols

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