![Western Blot: AMOT Antibody [NBP1-78210]](https://resources.rndsystems.com/images/products/AMOT-Antibody-Western-Blot-NBP1-78210-img0002.jpg "Western Blot: AMOT Antibody [NBP1-78210]")
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1034 and 1084 of human Angiomotin using the numbering given in entry NP_001106962.1 (GeneID 154796).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AMOT Antibody
Western Blot: AMOT Antibody [NBP1-78210]
Western Blot: AMOT Antibody [NBP1-78210] - Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-Angiomotin antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Angiomotin was also immunoprecipitated by rabbit anti-Angiomotin antibodies NBP1-78208 and NBP1-78209, which recognize upstream epitopes.Applications for AMOT Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 mcg/mg
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Angiomotin
Additional Angiomotin Products
Product Documents for AMOT Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AMOT Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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