Angiopoietin-4 Antibody (RM0073-2H34) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-21700

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # RM0073-2H34

Format

Azide and BSA Free
View all Angiopoietin-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse recombinant Angiopoietin-3

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for Angiopoietin-4 Antibody (RM0073-2H34) - Azide and BSA Free

Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody (RM0073-2H34) - Azide and BSA Free [NBP1-21700]

Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody (RM0073-2H34) - Azide and BSA Free [NBP1-21700]

Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody (RM0073-2H34) [NBP1-21700] - The Kidney tissue samples from the Folic Acid induced kidney injury model were fixed using 4% PFA at 4C for overnight and embeded in paraffin. 4um section was sublected to IHC with NBP1-21700

Applications for Angiopoietin-4 Antibody (RM0073-2H34) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-4

Angiopoietin 3 (ANG3), also called ANG4, might represent counterparts in mouse and human. Based on chromosomal analysis, mouse ANG3 is thought to be an ortholog of human ANG4. Human ANG4 acts as an agonist that binds and activates Tie2, whereas mouse ANG3 is likely to be a Tie2 antagonist, exerting agonist or antagonist activities depending on the cell context. Mouse ANG3 is expressed in various mouse tissues. Mouse angiopoietin 3 is a secreted glycoprotein and member of the angiopoietin family. It has an N terminal coiled-coil domain and a Cbterminal fibrinogen like domain. The coiled coil domains mediate ligand homo oligomerization and the fibrinogen like domains mediate ligand activity. Recombinant mouse angiopoietin 3 has a calculated molecular mass of 58 kDa. Due to glycosylation, the recombinant protein (containing 3 peptides) has an apparent molecular mass of 85-90 kDa in SDS-PAGE under reducing conditions. Mouse angiopoietin 3 shares 47%, 46%, and 54% sequence identity with mouse ANG1, mouse ANG2, and human ANG4, respectively.

Alternate Names

ANGPT4

Entrez Gene IDs

51378 (Human)

Gene Symbol

ANGPT4

Additional Angiopoietin-4 Products

Product Documents for Angiopoietin-4 Antibody (RM0073-2H34) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Angiopoietin-4 Antibody (RM0073-2H34) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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