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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (95%), Porcine (96%), Rabbit (95%), Sheep (97%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human ANKRD1. The exact sequence is proprietary.
Reactivity Notes
Rat reactivity reported from a verified customer review.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit ANKRD1 Antibody (NBP2-15397) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-ANKRD1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ANKRD1 Antibody
Western Blot: ANKRD1 Antibody [NBP2-15397]
Western Blot: ANKRD1 Antibody [NBP2-15397] - Analysis of rat primary neonatal ventricular cardiomyocytes using ANKRD1 antibody. Image from verified customer review.Immunocytochemistry/ Immunofluorescence: ANKRD1 Antibody [NBP2-15397]
Immunocytochemistry/Immunofluorescence: ANKRD1 Antibody [NBP2-15397] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.Western Blot: ANKRD1 Antibody [NBP2-15397]
Western Blot: ANKRD1 Antibody [NBP2-15397] - Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE; antibody diluted at 1:1000.Western Blot: ANKRD1 Antibody [NBP2-15397]
Western Blot: ANKRD1 Antibody [NBP2-15397] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE; antibody diluted at 1:1000.Applications for ANKRD1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 4 using NBP2-15397 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ANKRD1
Alternate Names
ALRP, ankyrin repeat domain 1 (cardiac muscle), ankyrin repeat domain-containing protein 1, bA320F15.2, C193, C-193, Cardiac ankyrin repeat protein, CARPCytokine-inducible nuclear protein, CVARP, Cytokine-inducible gene C-193 protein, HA1A2, liver ankyrin repeat domain 1, MCARP
Gene Symbol
ANKRD1
UniProt
Additional ANKRD1 Products
Product Documents for ANKRD1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ANKRD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for ANKRD1 Antibody
Customer Reviews for ANKRD1 Antibody (1)
4 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: primary neonatal ventricular cardiomyocytesSpecies: RatVerified Customer | Posted 11/28/2022Ankrd1 blot with rat primary cultured cardiomyocytes
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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