ANP32A Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99913

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ANP32A Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human ANP32A (Accession#: NP_006296.1; Glu2-Lys238).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ANP32A Antibody - Azide and BSA Free

Western Blot: ANP32A Antibody [NBP2-99913]

Western Blot: ANP32A Antibody [NBP2-99913]

Western Blot: ANP32A Antibody [NBP2-99913] - Lane A: ANP32A konckout Hela Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:28 kDa
Immunohistochemistry-Paraffin: ANP32A Antibody [NBP2-99913]

Immunohistochemistry-Paraffin: ANP32A Antibody [NBP2-99913]

Immunohistochemistry-Paraffin: ANP32A Antibody [NBP2-99913] - Staining of human ANP32A in human skeletal muscle with rabbit polyclonal antibody (1:1000).
Western Blot: ANP32A Antibody [NBP2-99913]

Western Blot: ANP32A Antibody [NBP2-99913]

Western Blot: ANP32A Antibody [NBP2-99913] - Lane A: HEK293 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:28 kDa
Immunohistochemistry-Paraffin: ANP32A Antibody [NBP2-99913]

Immunohistochemistry-Paraffin: ANP32A Antibody [NBP2-99913]

Immunohistochemistry-Paraffin: ANP32A Antibody [NBP2-99913] - Staining of human ANP32A in human kidney with rabbit polyclonal antibody (1:1000).
Immunoprecipitation: ANP32A Antibody [NBP2-99913]

Immunoprecipitation: ANP32A Antibody [NBP2-99913]

Immunoprecipitation: ANP32A Antibody [NBP2-99913] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ANP32A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ANP32A rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size: 29 kDa
ANP32A Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: ANP32A Antibody [NBP2-99913] -

Immunocytochemistry/Immunofluorescence: ANP32A Antibody [NBP2-99913] - Immunofluorescence staining of ANP32A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99913 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.

Applications for ANP32A Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANP32A

Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression

Alternate Names

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A, acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32, cerebellar leucine rich acidic nuclear protein, I1PP2A, inhibitor-1 of protein phosphatase-2A, LANPPutative HLA-DR-associated protein I, Leucine-rich acidic nuclear protein, MAPMC15orf1, mapmodulin, PHAP1MGC150373, PHAPIMGC119787, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, PP32, putative human HLA class II associated protein I

Gene Symbol

ANP32A

Additional ANP32A Products

Product Documents for ANP32A Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ANP32A Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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