Human/Mouse Phospho- Histone H2AX (S139) Antibody

R&D Systems | Catalog # 4418-APC-020

R&D Systems
Discontinued Product
4418-APC-020 has been discontinued. An alternative/replacement product is available: AF2288. View all Histone H2AX products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Frog - Xenopus (African Clawed Frog), Plant - Arabidopsis thaliana, Xenopus

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Histone H2AX Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human Histone H2AX S139 site

Specificity

Detects human and mouse Histone H2AX when phosphorylated at S139 in Western blots. This antibody detects only double-strand DNA breaks.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse Phospho- Histone H2AX (S139) Antibody

Phospho-Histone H2AX (S139) in C2C12 Mouse Cell Line.

Phospho-Histone H2AX (S139) was detected in immersion fixed C2C12 mouse myoblast cell line stimulated with etoposide (positive, left panel) and C2C12 mouse myoblast cell line (negative, right panel) using Rabbit Anti-Human/Mouse Phospho- Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # 4418-APC-020) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Phospho-Histone H2AX (S139) in Western blot.

Wehi cells were treated with (T)and without (H) 25 μM etoposide for 4 hours.Cells were lysed in Tris-Glycine SDS samplebuffer at the concentration 1 x 107cells/ml and10 μl of each lysate were loaded per well of 4-20% Tris-Glycine gel. Proteins were transferredonto an Immobilon FL membrane and Phospho-H2AX (S139)was detected with anti-phosphorylated HistoneH2AX antibody (Cat# 4418-APC-020, 1:2500) followed by IR800-conjugated anti-rabbit secondary antibody.

Applications for Human/Mouse Phospho- Histone H2AX (S139) Antibody

Application
Recommended Usage

Immunocytochemistry

1.7-15 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line stimulated with etoposide

Western Blot

1:2500 dilution
Sample: . Wehi cells were treated with etoposide

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution containing PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Histone H2AX

Histone H2AX is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed ( gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.

Alternate Names

H2AFX

Entrez Gene IDs

3014 (Human)

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Human/Mouse Phospho- Histone H2AX (S139) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Phospho- Histone H2AX (S139) Antibody

For research use only

Related Research Areas

Epigenetics

Citations for Human/Mouse Phospho- Histone H2AX (S139) Antibody

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Protocols

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