AP-2 gamma Antibody

Novus Biologicals | Catalog # NB100-1516

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AP-2 gamma Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NKTLEKMEKHRK corresponding to C-Terminus according to NP_003213.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for AP-2 gamma Antibody

Immunohistochemistry-Paraffin: AP-2 gamma Antibody [NB100-1516]

Immunohistochemistry-Paraffin: AP-2 gamma Antibody [NB100-1516]

Immunohistochemistry-Paraffin: AP-2 gamma Antibody [NB100-1516] - Staining of AP-2 gamma in paraffin embedded Human Skin using NB100-1516 at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: AP-2 gamma Antibody [NB100-1516]

Immunohistochemistry-Paraffin: AP-2 gamma Antibody [NB100-1516]

Immunohistochemistry-Paraffin: AP-2 gamma Antibody [NB100-1516] - (4ug/ml) Staining of Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Applications for AP-2 gamma Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 3 ug/ml

Immunohistochemistry-Paraffin

2 - 3 ug/ml

Peptide ELISA

Detection limit 1:128000
Application Notes
WB: WB experiments gave bands at approx. 75 kDa band and 22 kDa band in human placenta and ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2 kDa band according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human skin shows nuclear staining of keratinocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AP-2 gamma

The protein encoded by this gene is a sequence-specific DNA-binding transcription factor involved in the activation of several developmental genes. The encoded protein can act as either a homodimer or heterodimer with other family members and is induced during retinoic acid-mediated differentiation. It plays a role in the development of the eyes, face, body wall, limbs, and neural tube.

Long Name

Activating Enhancer-binding Protein gamma

Alternate Names

AP2 gamma, ERF1, TFAP2C, TFAP2G

Entrez Gene IDs

7022 (Human); 21420 (Mouse); 362280 (Rat)

Gene Symbol

TFAP2C

UniProt

Q92754

Additional AP-2 gamma Products

Product Documents for AP-2 gamma Antibody

Certificate of Analysis

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Product Specific Notices for AP-2 gamma Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

p53 Pathway

Citations for AP-2 gamma Antibody

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Protocols

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