APC Antibody (8X7Q1)
Novus Biologicals | Catalog # NBP3-15561
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8X7Q1 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human APC (NP_000029.2). MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSKMSLRSYGSRE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for APC Antibody (8X7Q1)
Western Blot: APC Antibody (8X7Q1) [NBP3-15561]
Western Blot: APC Antibody (8X7Q1) [NBP3-15561] - Western blot analysis of extracts of various cell lines, using APC antibody (NBP3-15561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunoprecipitation: APC Antibody (8X7Q1) [NBP3-15561]
Immunoprecipitation: APC Antibody (8X7Q1) [NBP3-15561] - Immunoprecipitation analysis of 600ug extracts of Mouse lung using 3ug APC antibody (NBP3-15561). Western blot was performed from the immunoprecipitate using APC antibody (NBP3-15561) at a dilition of 1:500.Applications for APC Antibody (8X7Q1)
Application
Recommended Usage
Immunoprecipitation
1:100 - 1:500
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: APC
Long Name
Adenomatosis Polyposis Coli
Alternate Names
DP2, DP3, FPC, GS, Min
Gene Symbol
APC
Additional APC Products
Product Documents for APC Antibody (8X7Q1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for APC Antibody (8X7Q1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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