APE Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56359

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all APE Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptides corresponding to amino acids 36-52 and 216-232 of human APE.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APE Antibody - BSA Free

Western Blot: APE Antibody [NB100-56359]

Western Blot: APE Antibody [NB100-56359]

Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.
Immunohistochemistry-Paraffin: APE Antibody [NB100-56359]

Immunohistochemistry-Paraffin: APE Antibody [NB100-56359]

Immunohistochemistry-Paraffin: APE Antibody [NB100-56359] - Human Breast: FFPE tissue section using APE antibody at 10 ug/mL.

Applications for APE Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 ug/mL

Western Blot

2 - 5 ug/mL

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APE

Human apurinic (apyrimidinic) endonuclease/redox-factor 1 (APE/Ref-1) is involved in the repair of DNA damage as well as in the transcriptional regulation of genes. The repair function of the protein is located on its C-terminal region. Activation of transcription factors, which occurs via a redox-based mechanism, pertains to a approximately 6 kDa N-terminal fragment with Cys-65 being of essential importance. Ape1/ref-1 has been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression such as Fos, Jun, NF (B, PAX, HIF-1(, HLF and p53. APE/Ref-1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents. Thus, specific downmodulation of APE/Ref-1 activity in tumors might be considered as a novel therapeutic approach in tumor therapy.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Entrez Gene IDs

328 (Human)

Gene Symbol

APEX1

UniProt

NP_001632 (Human)

Additional APE Products

Product Documents for APE Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for APE Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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Protocols

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FAQs

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