Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30393

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Apolipoprotein A-I/ApoA1 Primary Antibodies »

Product Specifications

Immunogen

Human Apolipoprotein AI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Apolipoprotein A-I/ApoA1 Antibody

Western Blot:[NBP3-30393]-

Western Blot:[NBP3-30393]-Detection of human ApoAI by western blot. Samples: Whole cell lysate from Hep-G2, MCF10A, SR, Malme-3M, and PC-3 cells prepared using NETN lysis buffer. Antibody: Affinity Purified Rabbit anti-Human Apo AI [A03R] Antibody used for WB at 1 µg/ml. Secondary: HRP-conjugated Goat anti-Rabbit IgG Heavy and Light Chain Cross-Adsorbed Antibody. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:80,000

Western Blot

1:1000 - 1:80,000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

75 mM Sodium Phosphate, 75 mM NaCl, 0.5 mM EDTA, pH 7.2

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Obesity

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