Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-08947

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # APOA4/3372

Format

Azide and BSA Free
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Product Specifications

Immunogen

Human recombinant Apolipoprotein A-IV/ApoA4 protein fragment (around aa 239-371) (exact sequence is proprietary) (Uniprot: P06727)

Localization

Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07203).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free [NBP3-08947]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free [NBP3-08947]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-08947] - Formalin-fixed, paraffin-embedded human liver stained with Apolipoprotein A-IV/ApoA4 Mouse Monoclonal Antibody (APOA4/3372).
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free [NBP3-08947]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free [NBP3-08947]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-08947] - Formalin-fixed, paraffin-embedded human liver carcinoma stained with Apolipoprotein A-IV/ApoA4 Mouse Monoclonal Antibody (APOA4/3372).
Protein Array: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free [NBP3-08947]

Protein Array: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free [NBP3-08947]

Protein Array: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-08947] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Apolipoprotein A-IV/ApoA4 Mouse Monoclonal Antibody (APOA4/3372).

Applications for Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Apolipoprotein A-IV/ApoA4

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Alternate Names

Apolipoprotein AIV

Gene Symbol

APOA4

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) - Azide and BSA Free

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  • Q: I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?

    A:

    All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

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