Apolipoprotein M/ApoM Antibody (1F10) - Azide and BSA Free

Novus Biologicals | Catalog # H00055937-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Sandwich ELISA, Dot Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F10

Format

Azide and BSA Free
View all Apolipoprotein M/ApoM Primary Antibodies »

Product Specifications

Immunogen

APOM (AAH20683, 23 a.a. ~ 188 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTKEELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKDGLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSSCPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDSKAFLLTPRNQEACELSNN

Reactivity Notes

Human. Other species not tested.

Specificity

APOM - apolipoprotein M

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Apolipoprotein M/ApoM Antibody (1F10) - Azide and BSA Free

Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01]

Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01]

Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Analysis of APOM expression in transfected 293T cell line by APOM monoclonal antibody (M01), clone 1F10.Lane 1: APOM transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.
Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01]

Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01]

Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Analysis of APOM over-expressed 293 cell line, cotransfected with APOM Validated Chimera RNAi ( Cat # H00055937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with APOM monoclonal antibody (M01), clone 1F10 (Cat # H00055937-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Detection limit for recombinant GST tagged APOM is approximately 0.3ng/ml as a capture antibody.

Applications for Apolipoprotein M/ApoM Antibody (1F10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Apolipoprotein M/ApoM

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized.

Alternate Names

APOM, NG20, Protein G3a

Entrez Gene IDs

55937 (Human)

Gene Symbol

APOM

OMIM

606907 (Human)

UniProt

AAH20683 (Human)

Additional Apolipoprotein M/ApoM Products

Product Documents for Apolipoprotein M/ApoM Antibody (1F10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Apolipoprotein M/ApoM Antibody (1F10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

Citations for Apolipoprotein M/ApoM Antibody (1F10) - Azide and BSA Free

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