Q: I was looking for antibodies against human ARF4 and ARF5 and I need to be able to distinguish between the two proteins. Do you have antibodies specific for each that don't cross react with the other protein?

A: The sequence similarity between ARF4 and ARF5 shows very high homology between the two proteins. We have not specifically tested for cross-reactivity between the antibodies, but I think it's very likely that they will cross react.

ARF4 Antibody

Novus Biologicals | Catalog # NBP3-12381

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

View all ARF4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on ARF4 protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for ARF4 Antibody

Western Blot: ARF4 Antibody [NBP3-12381]

Western Blot: ARF4 Antibody [NBP3-12381] - Various mouse tissues and ARF4 positive control. 1) Rat hippocampus, 2) Rat lung, 3) PC-ARF4, 4) Rat spleen, 5) Rat kidney. 1:250 dilution in Diluobuffer. Apparent MW is 22 KDa.

Applications for ARF4 Antibody

Application

Recommended Usage

ELISA

1:10000

Immunoprecipitation

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: ARF4

The ARF4 gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins thatstimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and asactivators of phospholipase

Alternate Names

ADP-ribosylation factor 4, ARF2ADP-ribosylation factor 2

Gene Symbol

ARF4

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Product Documents for ARF4 Antibody

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Certificate of Analysis

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Product Specific Notices for ARF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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