ARFGAP3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31064

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (92%), Chicken (92%), Mouse (92%), Primate (100%), Rat (92%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ARFGAP3 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ARFGAP3. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (85%), Xenopus Tropicalis (85%), Rhesus Monkey (100%).

Localization

Cytoplasm, Golgi apparatus membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARFGAP3 Antibody - BSA Free

Western Blot: ARFGAP3 Antibody [NBP1-31064]

Western Blot: ARFGAP3 Antibody [NBP1-31064]

Western Blot: ARFGAP3 Antibody [NBP1-31064] - Sample(30 ug of whole cell lysate)A:293T7. 5% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: ARFGAP3 Antibody [NBP1-31064]

Immunocytochemistry/ Immunofluorescence: ARFGAP3 Antibody [NBP1-31064]

Immunocytochemistry/Immunofluorescence: ARFGAP3 Antibody [NBP1-31064] - Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: ARFGAP3 protein stained by ARFGAP3 antibody, C-term diluted at 1:500. Blue: Hoechst 33343 staining.

Applications for ARFGAP3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARFGAP3

The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq]

Alternate Names

ADP-ribosylation factor GTPase activating protein 1, ADP-ribosylation factor GTPase activating protein 3, ADP-ribosylation factor GTPase-activating protein 3, ARF GAP 3, ARFGAP1, FLJ45618

Gene Symbol

ARFGAP3

Additional ARFGAP3 Products

Product Documents for ARFGAP3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ARFGAP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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