Arginase 1/ARG1/liver Arginase Antibody (BLR161J)

Novus Biologicals | Catalog # NBP3-14735

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR161J
View all Arginase 1/ARG1 Primary Antibodies »

Product Specifications

Immunogen

residues 1-322 (FL)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Arginase 1/ARG1/liver Arginase Antibody (BLR161J)

Immunoprecipitation: Arginase 1/ARG1/liver Arginase Antibody (BLR161J) [NBP3-14735]

Immunoprecipitation: Arginase 1/ARG1/liver Arginase Antibody (BLR161J) [NBP3-14735]

Immunoprecipitation: Arginase 1/ARG1/liver Arginase Antibody [NBP3-14735] - Tissue lysate (1.0 mg per IPreaction; 20% of IP loaded) from mouse liver cells preparedusing NETN lysis buffer. Antibody: Rabbit anti-Arginase 1recombinant monoclonal antibody used at 20 ul/mg lysate. For blottingimmunoprecipitated Arginase 1, was used at1:1000. Chemiluminescence with an exposure time of 30seconds.
Arginase 1/ARG1/liver Arginase Antibody (BLR161J)

Arginase 1/ARG1/liver Arginase Antibody [NBP3-14735] -

Lysate from from H-4-II-E, NIH 3T3, mouse liver, TCMK-1,and AML12 cells. Antibody: Rabbit anti-Arginase-1recombinant monoclonal antibody [BLR161J] used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with anexposure time of 30 seconds. Lower Panel: Rabbit antiActin recombinant monoclonal antibody [BLR057F]
Arginase 1/ARG1/liver Arginase Antibody (BLR161J)

Arginase 1/ARG1/liver Arginase Antibody [NBP3-14735] -

Rabbit anti-Arginase 1 recombinant monoclonal antibody [BLR161J]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.

Applications for Arginase 1/ARG1/liver Arginase Antibody (BLR161J)

Application
Recommended Usage

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

20 µl/1 mg lysate

Western Blot

1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Arginase 1/ARG1

Arginase 1 (ARG1), also known as liver arginase, is a metalloenzyme that is a member of the ureohydrolase superfamily and arginase family (1). ARG1 is known for its role in the urea cycle in catalyzing the conversion of L-arginine into urea and L-ornithine (1). Arginase has two distinct isoforms, with ARG1 being expressed primarily in the liver and ARG2 in extrahepatic tissues (1). Human ARG1 is synthesized as 322 amino acids (aa) in length with a theoretical molecular weight of 35 kDa (1,2). Three ARG1 monomers can form a highly active homotrimer of 105 kDa (1). A key structural feature of the arginase protein is the binuclear magnesium (Mn2+) ions at its core (1).

Arginase and nitric oxidase synthase (NOS) compete for the same L-arginine substrate, creating a delicate balance between pathways (1). Furthermore, bioavailability of L-arginine and ARG1 expression has been implicated in several pathologies including vascular disease, neuronal disease, cardiovascular disease, immune dysfunction, inflammation, and cancer (1,3-5). For instance, ARG1 functions as a macrophage marker, defining the M2 population, while inducible nitric oxide synthase (iNOS) characterizes the M1 population; impaired M1/M2 polarization and changes in ARG1 expression is observed in diseases such as arteriogenesis, asthma, pulmonary fibrosis, and inflammatory bowel disease (1,3). In humans, arginase deficiency, known as argininemia, is an autosomal recessive metabolic disorder characterized by elevated ammonia (hyperammonemia) levels and arginine accumulation (6). Given that many arginase-associated diseases are characterized by upregulation in expression of ARG1, ARG2, or both, arginase inhibitors are currently being studied as a potential therapeutic approach (1,4).

References

1. S Clemente, G., van Waarde, A., F Antunes, I., Domling, A., & H Elsinga, P. (2020). Arginase as a Potential Biomarker of Disease Progression: A Molecular Imaging Perspective. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21155291

2. Uniprot (P05089)

3. Kieler, M., Hofmann, M., & Schabbauer, G. (2021). More than just protein building blocks: How amino acids and related metabolic pathways fuel macrophage polarization. The FEBS Journal. Advance online publication. https://doi.org/10.1111/febs.15715

4. Shosha, E., Fouda, A. Y., Narayanan, S. P., Caldwell, R. W., & Caldwell, R. B. (2020). Is the Arginase Pathway a Novel Therapeutic Avenue for Diabetic Retinopathy?. Journal of Clinical Medicine. https://doi.org/10.3390/jcm9020425

5. Correale J. (2021). Immunosuppressive Amino-Acid Catabolizing Enzymes in Multiple Sclerosis. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.600428

6. Morales, J. A., & Sticco, K. L. (2020). Arginase Deficiency. In StatPearls. StatPearls Publishing.

Long Name

Liver-Type Arginase

Alternate Names

AI, ARG1, Arginase-1, Liver Arginase, PGIF, Type I Arginase

Gene Symbol

ARG1

Additional Arginase 1/ARG1 Products

Product Documents for Arginase 1/ARG1/liver Arginase Antibody (BLR161J)

Certificate of Analysis

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Product Specific Notices for Arginase 1/ARG1/liver Arginase Antibody (BLR161J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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