Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70204

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E6

Format

Azide and BSA Free
View all Arginase 1/ARG1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ARG1 (NP_000036) produced in HEK293T cell.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31575880). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

34.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6)Azide and BSA Free [NBP2-70204]

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6)Azide and BSA Free [NBP2-70204]

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver A
Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204]

Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204]

Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Staining of paraffin-embedded Human liver tissue using anti-liver Arginase mouse monoclonal antibody.
Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204]

Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204]

Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-liver Arginase antibody, and then analyzed by flow cytometry.
Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6)Azide and BSA Free [NBP2-70204]

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6)Azide and BSA Free [NBP2-70204]

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiARG1 monoclonal antibody.(1:200)
Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6)Azide and BSA Free [NBP2-70204]

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6)Azide and BSA Free [NBP2-70204]

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Analysis of extracts (35ug) from 9 different cell lines by using anti-liver Arginase monoclonal antibody.

Applications for Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Arginase 1/ARG1

Arginase 1 (ARG1), also known as liver arginase, is a metalloenzyme that is a member of the ureohydrolase superfamily and arginase family (1). ARG1 is known for its role in the urea cycle in catalyzing the conversion of L-arginine into urea and L-ornithine (1). Arginase has two distinct isoforms, with ARG1 being expressed primarily in the liver and ARG2 in extrahepatic tissues (1). Human ARG1 is synthesized as 322 amino acids (aa) in length with a theoretical molecular weight of 35 kDa (1,2). Three ARG1 monomers can form a highly active homotrimer of 105 kDa (1). A key structural feature of the arginase protein is the binuclear magnesium (Mn2+) ions at its core (1).

Arginase and nitric oxidase synthase (NOS) compete for the same L-arginine substrate, creating a delicate balance between pathways (1). Furthermore, bioavailability of L-arginine and ARG1 expression has been implicated in several pathologies including vascular disease, neuronal disease, cardiovascular disease, immune dysfunction, inflammation, and cancer (1,3-5). For instance, ARG1 functions as a macrophage marker, defining the M2 population, while inducible nitric oxide synthase (iNOS) characterizes the M1 population; impaired M1/M2 polarization and changes in ARG1 expression is observed in diseases such as arteriogenesis, asthma, pulmonary fibrosis, and inflammatory bowel disease (1,3). In humans, arginase deficiency, known as argininemia, is an autosomal recessive metabolic disorder characterized by elevated ammonia (hyperammonemia) levels and arginine accumulation (6). Given that many arginase-associated diseases are characterized by upregulation in expression of ARG1, ARG2, or both, arginase inhibitors are currently being studied as a potential therapeutic approach (1,4).

References

1. S Clemente, G., van Waarde, A., F Antunes, I., Domling, A., & H Elsinga, P. (2020). Arginase as a Potential Biomarker of Disease Progression: A Molecular Imaging Perspective. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21155291

2. Uniprot (P05089)

3. Kieler, M., Hofmann, M., & Schabbauer, G. (2021). More than just protein building blocks: How amino acids and related metabolic pathways fuel macrophage polarization. The FEBS Journal. Advance online publication. https://doi.org/10.1111/febs.15715

4. Shosha, E., Fouda, A. Y., Narayanan, S. P., Caldwell, R. W., & Caldwell, R. B. (2020). Is the Arginase Pathway a Novel Therapeutic Avenue for Diabetic Retinopathy?. Journal of Clinical Medicine. https://doi.org/10.3390/jcm9020425

5. Correale J. (2021). Immunosuppressive Amino-Acid Catabolizing Enzymes in Multiple Sclerosis. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.600428

6. Morales, J. A., & Sticco, K. L. (2020). Arginase Deficiency. In StatPearls. StatPearls Publishing.

Long Name

Liver-Type Arginase

Alternate Names

AI, ARG1, Arginase-1, Liver Arginase, PGIF, Type I Arginase

Gene Symbol

ARG1

Additional Arginase 1/ARG1 Products

Product Documents for Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free and earn rewards!

Have you used Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies