ARX Antibody

Novus Biologicals | Catalog # NBP2-80506

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all ARX Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 2-50 of human ARX conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for ARX Antibody

Western Blot: ARX Antibody [NBP2-80506]

Western Blot: ARX Antibody [NBP2-80506]

Western Blot: ARX Antibody [NBP2-80506] - WB on mouse brain lysate. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:1500 dilution incubated overnight at 4C.
Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Applications for ARX Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2000

Immunohistochemistry-Paraffin

1:1000 - 1:2000

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Formulation

Lyophilized from whole serum

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ARX

Arx is a homeobox-containing gene expressed during development. The expressed protein contains two conserved domains, a C-peptide (or aristaless domain) and the prd-like class homeobox domain. It is a member of the group-II aristaless-related protein family whose members are expressed primarily in the central and/or peripheral nervous system. This gene is thought to be involved in CNS development. Mutations in this gene cause X-linked mental retardation and epilepsy.

Long Name

Aristless-related Homeobox Protein

Alternate Names

CT121, EIEE1, ISSX, MRX29, MRX32, MRX33, MRX36, MRX38, MRX43, MRX54, MRX76, MRX87, MRXS1, PRTS

Gene Symbol

ARX

Additional ARX Products

Product Documents for ARX Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ARX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ARX Antibody

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  • Q: NBP2-80506, I had brought this antibody, but I cannot find the concentration of the antibody.

    A: This ARX antibody is unpurified, as it was extracted from whole serum. The exact concentration of antibody is not quantifiable.

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