Arylsulfatase A/ARSA Antibody

Novus Biologicals | Catalog # NBP1-00154

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Arylsulfatase A/ARSA Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YDLSKDPGENYN corresponding to internal region according to NP_000478.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Arylsulfatase A/ARSA Antibody

Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Western blot analysis of Mouse testes lysate (A) + peptide (B) with antibody at 1 ug/mL and Rat testes lysate (C) + peptide (D) with antibody at 0.3 ug/mL, 35ug protein in RIPA buffer. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Immunocytochemistry/ Immunofluorescence: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Immunocytochemistry/Immunofluorescence: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Flow Cytometry: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Flow Cytometry: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Arylsulfatase A/ARSA Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
Approx 60-65 kDa band observed in Mouse and Rat Testis lysates (calculated MW of 53.7 kDa according to Mouse NP_033843.2 and Rat NP_001030105.2;). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Arylsulfatase A/ARSA

The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Multiple alternatively spliced transcript variants, one of which encodes a distinct protein, have been described for this gene. [provided by RefSeq]

Alternate Names

ARSA, Cerebroside 3-sulfatase

Entrez Gene IDs

410 (Human); 11883 (Mouse); 315222 (Rat)

Gene Symbol

ARSA

UniProt

NP_000478.2 (Human)

Additional Arylsulfatase A/ARSA Products

Product Documents for Arylsulfatase A/ARSA Antibody

Certificate of Analysis

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Product Specific Notices for Arylsulfatase A/ARSA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Arylsulfatase A/ARSA Antibody

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Protocols

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