Asparagine synthetase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-56407

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Asparagine Synthetase/ASNS Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVDPLFGMQPIRVKKYPYLWLCYNGEIYNHKKMQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Asparagine synthetase Antibody - BSA Free

Western Blot: Asparagine synthetase Antibody [NBP2-56407]

Western Blot: Asparagine synthetase Antibody [NBP2-56407]

Western Blot: Asparagine synthetase Antibody [NBP2-56407] - Analysis in human cell line U-87 MG.
Immunocytochemistry/ Immunofluorescence: Asparagine synthetase Antibody [NBP2-56407]

Immunocytochemistry/ Immunofluorescence: Asparagine synthetase Antibody [NBP2-56407]

Immunocytochemistry/Immunofluorescence: Asparagine synthetase Antibody [NBP2-56407] - Staining of human cell line MCF7 shows localization to cytosol. Antibody staining is shown in green.

Applications for Asparagine synthetase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04 - 0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Asparagine Synthetase/ASNS

Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).

Long Name

ASNS

Alternate Names

ASNSD, EC 6.3.5.4, Glutamine Dependent Asparagine Synthetase, TS11 Cell Cycle Control Protein

Gene Symbol

ASNS

Additional Asparagine Synthetase/ASNS Products

Product Documents for Asparagine synthetase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Asparagine synthetase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

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Protocols

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FAQs

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